Recombinant hMART-1 protein (A103), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Alternative Names:
Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (2-4ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 min
Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).
Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).
Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).
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