This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
SDS-PAGE Analysis of Purified MSA Recombinant Mouse Monoclonal Antibody (rMSA/953). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of human skeletal muscle tissue lysate using Muscle Specific Actin Recombinant Mouse Monoclonal Antibody (rMSA/953).
Western blot analysis of human intestine and human skeletal muscle tissue lysates using Muscle Specific Actin Recombinant Mouse Monoclonal Antibody (rMSA/953).
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