Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, IgG1, Clone: [Cocktail rPCK/6750], Mouse, Monoclonal

Article Name:	Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, IgG1, Clone: [Cocktail rPCK/6750], Mouse, Monoclonal
Biozol Catalog Number:	NBT-MSM50-6750-P0
Supplier Catalog Number:	MSM50-6750-P0
Alternative Catalog Number:	NBT-MSM50-6750-P0-20,NBT-MSM50-6750-P0-100
Manufacturer:	NeoBiotechnologies
Host:	Mouse
Category:	Antikörper
Application:	FC, IF, IHC, WB
Species Reactivity:	Canine, Feline, Human
Immunogen:	Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
Alternative Names:	K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Anti-cytokeratin clone rPCK/6750 (CK rPCK/6750) demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Clonality:	Monoclonal
Clone Designation:	[Cocktail rPCK/6750]
Molecular Weight:	40-67kDa (Multiple)
Isotype:	IgG1
NCBI:	Multiple
Form:	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Recombinant Monoclonal Antibody

Application Notes:

Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min

	SDS-PAGE Analysis of Purified Pan CK Recombinant Mouse Monoclonal Antibody (rPCK/6750). Confirmation of Purity and Integrity of Antibody.
	Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Recombinant Mouse Monoclonal Antibody (rPCK/6750).
	Western Blot Analysis of Human Skin and A431 lysates using Pan Cytokeratin Recombinant Mouse Monoclonal Antibody (-i-+--ö/6750).