This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Clonality:
Monoclonal
Clone Designation:
[rNM106]
Molecular Weight:
Not Known
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mi
SDS-PAGE Analysis of Purified Pan-Nuclear AntigenRecombinant Mouse Monoclonal (rNM106). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Heart, Human Intestine, Human Liver and Human Skeletal muscle tissue lysates using Nuclear Antigen Recombinant Mouse Monoclonal Antibody (rNM106).
* VAT and and shipping costs not included. Errors and price changes excepted
