It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality:
Monoclonal
Clone Designation:
[BTN/2032R]
Molecular Weight:
244Da
NCBI:
Not Applicable
Form:
200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
In situ Nucleic Acid Hybridization, Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a speci
SDS-PAGE Analysis of Purified anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2
Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
SDS-PAGE Analysis of Purified anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R). Confirmation of Purity and Integrity of Antibody.
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