STAT1 alpha Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	STAT1 alpha Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-1021
Supplier Catalog Number:	1021
Alternative Catalog Number:	PRS-1021-0.02,PRS-1021-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IF, IHC-P, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-STAT1 alpha antibody (1021) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha.
Conjugation:	Unconjugated
Alternative Names:	STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91

Clonality:	Polyclonal
Concentration:	1 mg/ml
Molecular Weight:	Predicted: 87kDObserved: 87 kD
NCBI:	6772
UniProt:	P42224
Buffer:	STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-1 µg/mL, IF: 20 µg/mL, IHC: 2.5 µg/mL, ICC: 10 µg/mL .Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence, Immunohistochemistry and Immunocytochemistry in human samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH Loading: 15 &956,g of lysates per lane.Antibodies: STAT1 alpha 1021 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 7 Immunocytochemistry Validation of STAT1 alpha in Human HeLa CellsImmunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (1021) at 10 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 5 Immunofluorescence Validation of STAT1 alpha in Human Colon TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with 1021 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (1021) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: STAT1 alpha 1021 (1 &956,g/mL), competitor antibody (2 &956,g/mL), and beta-actin (1.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Mouse tissues Loading: 15 &956,g of lysates per lane.Antibodies: STAT1 alpha 1021 (0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Rat Cell Line Loading: 15 &956,g of lysates per lane.Antibodies: STAT1 alpha 1021 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.