NeuN Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-13-332
- Images (6)
| Article Name: | NeuN Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-13-332 |
| Supplier Catalog Number: | 13-332 |
| Alternative Catalog Number: | PRS-13-332-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | FOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using NeuN antibody (13-332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
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Western blot analysis of extracts from normal (control) and NeuN knockout (KO) 293T cells, using NeuN antibody (13-332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
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Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody (13-332) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody (13-332) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using NeuN antibody (13-332) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-251MG cells using NeuN antibody (13-332) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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