Hexokinase II Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-360
Article Name: Hexokinase II Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-360
Supplier Catalog Number: 13-360
Alternative Catalog Number: PRS-13-360-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Conjugation: Unconjugated
Alternative Names: HK2, DKFZp686M1669, HKII, HXK2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 102kDa
NCBI: 3099
UniProt: P52789
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Hexokinase II antibody (13-360) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Hexokinase II antibody (13-360). Blue: DAPI for nuclear staining.