IGF1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-216
Article Name: IGF1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-216
Supplier Catalog Number: 14-216
Alternative Catalog Number: PRS-14-216-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IGF1 (NP_000609.1).
Conjugation: Unconjugated
Alternative Names: IGF1, OTTHUMP00000195084, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor 1, IGFI, IGF1A, IBP1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 22kDa
NCBI: 3479
UniProt: P05019
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using IGF1 antibody (14-216) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).