PRPF19 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-527
Article Name: PRPF19 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-527
Supplier Catalog Number: 14-527
Alternative Catalog Number: PRS-14-527-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).
Conjugation: Unconjugated
Alternative Names: PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 55kDa
NCBI: 27339
UniProt: Q9UMS4
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PRPF19 antibody (14-527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PRPF19 antibody (14-527). Western blot was performed from the immunoprecipitate using PRPF19 antibody (14-527) at a dilition of 1:1000.