EIF2AK4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-545
Article Name: EIF2AK4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-545
Supplier Catalog Number: 14-545
Alternative Catalog Number: PRS-14-545-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
Conjugation: Unconjugated
Alternative Names: EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, Mgcn2, KIAA1338
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 187kDa
NCBI: 440275
UniProt: Q9P2K8
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (14-545). Blue: DAPI for nuclear staining.