Gm13125 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-546
Article Name: Gm13125 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-546
Supplier Catalog Number: 14-546
Alternative Catalog Number: PRS-14-546-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1).
Conjugation: Unconjugated
Alternative Names: EG627009, Gm13125, novel preferentially expressed antigen in melanoma (Prame) protein
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 55kDa
NCBI: 627009
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Gm13125 antibody (14-546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).