Aromatase Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-578
Article Name: Aromatase Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-578
Supplier Catalog Number: 14-578
Alternative Catalog Number: PRS-14-578-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2).
Conjugation: Unconjugated
Alternative Names: CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1, ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 48kDa
NCBI: 1588
UniProt: P11511
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of HeLa cells, using Aromatase antibody (14-578) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon using Aromatase antibody (14-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Aromatase antibody (14-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using Aromatase antibody (14-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Aromatase antibody (14-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using Aromatase antibody (14-578) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Aromatase antibody (14-578) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.