BAG3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-15-750
Article Name: BAG3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-15-750
Supplier Catalog Number: 15-750
Alternative Catalog Number: PRS-15-750-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
Conjugation: Unconjugated
Alternative Names: BAG3, BCL2-associated athanogene 3 , BAG-3, BIS, CAIR-1, MGC104307, BAG-family molecular chaperone regulator-3, BCL2-binding athanogene 3, Bcl-2-binding protein, docking protein CAIR-1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 80kDa
NCBI: 9531
UniProt: O95817
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using BAG3 antibody (15-750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).