KRT14 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-15-857
Article Name: KRT14 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-15-857
Supplier Catalog Number: 15-857
Alternative Catalog Number: PRS-15-857-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
Conjugation: Unconjugated
Alternative Names: KRT14, K14, NFJ, CK14, EBS3, EBS4
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 56kDa
NCBI: 3861
UniProt: P02533
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Rat skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.