Western blot analysis of extracts of various cell lines, using S100A8 antibody (15-986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (15-986) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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