Acetyl-Histone H2B-K12 pAb, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-16-140
- Images (7)
| Article Name: | Acetyl-Histone H2B-K12 pAb, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-16-140 |
| Supplier Catalog Number: | 16-140 |
| Alternative Catalog Number: | PRS-16-140-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105 |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (16-140) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.C2C12 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s. |
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Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens). |
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Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
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