IL2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-16-547
Article Name: IL2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-16-547
Supplier Catalog Number: 16-547
Alternative Catalog Number: PRS-16-547-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human IL2
Conjugation: Unconjugated
Alternative Names: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 18kDa
NCBI: 3558
UniProt: P60568
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using IL2 antibody (16-547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (16-547) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).