Histone H3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-18-615
- Images (6)
| Article Name: | Histone H3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-18-615 |
| Supplier Catalog Number: | 18-615 |
| Alternative Catalog Number: | PRS-18-615-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Other, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | H3F3A, H3t, H3.4, H3/g, H3FT |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (18-615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunohistochemistry of paraffin-embedded human placenta using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (18-615) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (18-615) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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