MonoMethyl-Histone H4-K20 pAb, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-633
Article Name: MonoMethyl-Histone H4-K20 pAb, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-633
Supplier Catalog Number: 18-633
Alternative Catalog Number: PRS-18-633-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ChIP, IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Other, Rat
Immunogen: A synthetic peptide of human MonoMethyl-Histone H4-K20
Conjugation: Unconjugated
Alternative Names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 13kDa
NCBI: 8370
UniProt: P62805
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-K20 antibody (18-633).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H4-K20 antibody (18-633).
Immunohistochemistry of paraffin-embedded human colon using MonoMethyl-Histone H4-K20 antibody (18-633) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H4-K20 antibody (18-633) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MonoMethyl-Histone H4-K20 antibody (18-633) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 antibody (18-633). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H4-K20 antibody (18-633) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.