Occludin Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-18-733
- Images (6)
| Article Name: | Occludin Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-18-733 |
| Supplier Catalog Number: | 18-733 |
| Alternative Catalog Number: | PRS-18-733-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | Occludin, OCLN |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:1000 - 1:2000 |
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Western blot analysis of extracts of various cell lines, using Occludin antibody (18-733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
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Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (18-733) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (18-733) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC12 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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