COX5B Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-18-748
- Images (7)
| Article Name: | COX5B Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-18-748 |
| Supplier Catalog Number: | 18-748 |
| Alternative Catalog Number: | PRS-18-748-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2). |
| Conjugation: | Unconjugated |
| Alternative Names: | Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using COX5B antibody (18-748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s. |
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Immunohistochemistry of paraffin-embedded mouse heart using COX5B antibody (18-748) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human liver damage using COX5B antibody (18-748) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of C6 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH-3T3 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-2 OS cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug COX5B antibody (18-748). Western blot was performed from the immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000. |
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