ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-597
Article Name: ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-597
Supplier Catalog Number: 19-597
Alternative Catalog Number: PRS-19-597-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
Conjugation: Unconjugated
Alternative Names: acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 42kDa
NCBI: 38
UniProt: P24752
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ACAT1 antibody (19-597) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.