SCNN1A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-716
Article Name: SCNN1A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-716
Supplier Catalog Number: 19-716
Alternative Catalog Number: PRS-19-716-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).
Conjugation: Unconjugated
Alternative Names: Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, ENaCA, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN1A, SCNN1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 76kDa
NCBI: 6337
UniProt: P37088
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:200 - 1:2000IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SCNN1A antibody (19-716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.