FUBP1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-747
Article Name: FUBP1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-747
Supplier Catalog Number: 19-747
Alternative Catalog Number: PRS-19-747-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
Conjugation: Unconjugated
Alternative Names: FUBP1, FBP, FUBP
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 79kDa
NCBI: 8880
UniProt: Q96AE4
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using FUBP1 antibody (19-747) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FUBP1 antibody (19-747) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug FUBP1 antibody (19-747). Western blot was performed from the immunoprecipitate using FUBP1 antibody (19-747) at a dilition of 1:1000.