Western blot analysis of extracts of various cell lines, using FUBP1 antibody (19-747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FUBP1 antibody (19-747) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug FUBP1 antibody (19-747). Western blot was performed from the immunoprecipitate using FUBP1 antibody (19-747) at a dilition of 1:1000.
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