CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-768
Article Name: CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-768
Supplier Catalog Number: 19-768
Alternative Catalog Number: PRS-19-768-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
Conjugation: Unconjugated
Alternative Names: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 115kDa
NCBI: 1495
UniProt: P35221
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (19-768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody (19-768). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (19-768) at a dilition of 1:500.