Western blot analysis of extracts of various cell lines, using GNA13 antibody (19-832) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (19-832) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (19-832) (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (19-832) (40x lens).
Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (19-832) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (19-832) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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