p53 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-19-842
- Images (6)
| Article Name: | p53 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-19-842 |
| Supplier Catalog Number: | 19-842 |
| Alternative Catalog Number: | PRS-19-842-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 218-390 of mouse p53 (NP_035770.2). |
| Conjugation: | Unconjugated |
| Alternative Names: | TRP53, bbl, bfy, bhy, p44, p53, Tp53 |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 53kDa |
| NCBI: | 22059 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Form: | Liquid |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:3000 - 1:6000IHC: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50 |
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Western blot analysis of extracts of various cell lines, using p53 antibody (19-842) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s. |
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Immunohistochemistry of paraffin-embedded rat ovary using p53 antibody (19-842) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using p53 antibody (19-842) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human gastric cancer using p53 antibody (19-842) at dilution of 1:100 (40x lens). |
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Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug p53 antibody (19-842). Western blot was performed from the immunoprecipitate using p53 antibody (19-842) at a dilition of 1:500. |
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Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (19-842) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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