Caspase-9 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	Caspase-9 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-2071
Supplier Catalog Number:	2071
Alternative Catalog Number:	PRS-2071-0.02,PRS-2071-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9. The immunogen is located within amino acids 30-80 of Caspase 9.
Conjugation:	Unconjugated
Alternative Names:	Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9

Clonality:	Polyclonal
Concentration:	1 mg/ml
Molecular Weight:	Predicted: 46kDObserved: 46kD
NCBI:	842
UniProt:	P55211
Buffer:	Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-2 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071 (1 &956,g/mL), Caspase 9, 2073 (1 &956,g/mL) and beta-actin (1.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071 (1 &956,g/mL)), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human HeartLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 &956,g/mLLane 2: 2 &956,g/mLLane 3: 4 &956,g/mL
	Figure 4 Western Blot Validation in HeLa Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Western Blot Validation in Mouse Spleen TissueLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 &956,g/mLLane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL
	Figure 6 Western Blot Validation in Rat Spleen TissueLoading: 15 &956,g of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.