SET Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-055
Article Name: SET Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-055
Supplier Catalog Number: 22-055
Alternative Catalog Number: PRS-22-055-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human SET (NP_003002.2).
Conjugation: Unconjugated
Alternative Names: SET, SET translocation (myeloid leukemia-associated), 2PP2A, I2PP2A, PHAPII, TAF-IBETA, SET, translocation, IGAAD, inhibitor-2 of protein phosphatase-2A, Inhibitor of GZMA-activated DNAse, TAF-I, IPP2A2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
NCBI: 6418
UniProt: Q01105
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SET antibody (22-055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody (22-055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (22-055) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.