AIF Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	AIF Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-2239
Supplier Catalog Number:	2239
Alternative Catalog Number:	PRS-2239-0.02,PRS-2239-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, WB
Species Reactivity:	Human
Immunogen:	Anti-AIF antibody (2239) was raised against a peptide corresponding to 14 amino acids near the amino terminus of mature human AIF. The immunogen is located within amino acids 90-140 of AIF.
Conjugation:	Unconjugated
Alternative Names:	AIF Antibody: Apoptosis-inducing factor 1, Programmed cell death protein 8, AIF, PDCD8

Clonality:	Polyclonal
Concentration:	1 mg/ml
Molecular Weight:	Predicted: 67 kDa Observed: 68 kDa
NCBI:	9131
UniProt:	O95381
Buffer:	AIF Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 3 Immunofluorescence Validation of AIF in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling AIF with 2239 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
	Figure 6 Immunofluorescence Validation of AIF in Rat Hippocampal Neurons (Hofer et al., 2011)(G-L) After exposure to bacterial components, AIF colocalized in mature neurons (MAP2, I, L), immature neurons (DcX,H, K), and stem/progenitor cells (Nestin, G, J). AIF expression was detected by anti-AIF antibodies.
	Figure 7 Subcellular Localization Validation of AIF in mononuclear cells (Gupta et al., 2003) A shows mononuclear cells (MNCs) alone, B shows MNCs transfected with control plasmid, C shows MNCs transfected with Bcl-2 expression plasmid. Overlay is of Mitotracker (red) and AIF (green). Hoechst 33258 dye is used to examine chromatin fragmentation. The release of AIF form mitochondria is detected by anti-AIF antibodies.
	Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: AIF 2239, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: AIF 2267, (1 &956,g/mL), AIF 2239, (1 &956,g/mL), AIF 2301, (2 &956,g/mL), and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 KD and Induced Validation of AIF in H1299 Cells(Stambolsky et al., 2006) Western blot analysis of AIF knockdown with anti-AIF antibodies in H1299 cells. AIF expression was disrupted in AIF knockdown cells (siRNA1 and siRNA4). An increased expression of AIF was induced by ZnCl2 treatment, which was not observed in AIF knockdown cells.
	Figure 5 KD Validation of AIF in AIF Silenced Stable Cells(Apostolova et al., 2006) AIF silencing is sustained in stable cell lines. Western blot analysis ofstable lines AIF-1-10, AIF-2-4 and pU6-2 using anti-AIF antibodies. AIF protein was disrupted after AIF silencing with AIF siRNA (AIF-1-10 and AIF-2-4) as compared to control (Hep3B and pU6-2).
	Figure 8 Induced Expression Validation of AIF in U937 Cells (Ikai et al., 2006) Release of AIF at 48 h after the treatment with 30 uM magnolol examined by Western blotAnalysis with anti-AIF antibodies. AIF release was markedly increased 48h after magnolol treatment.