ASC Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	ASC Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-2287
Supplier Catalog Number:	2287
Alternative Catalog Number:	PRS-2287-0.02,PRS-2287-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Anti-ASC antibody (2287) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.
Conjugation:	Unconjugated
Alternative Names:	ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 22kDObserved: 22 kD
NCBI:	29108
UniProt:	Q9ULZ3
Buffer:	ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, ICC: 5 µg/mL, IHC-P: 2.5 µg/mL.Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 6 Immunocytochemistry Validation of ASC in HL60 CellsImmunocytochemical analysis of HL60 cells using anti-ASC antibody (2287) at 5 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 5 Immunofluorescence Validation of ASC in Human Spleen TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with 2287 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 4 Immunohistochemistry Validation of ASC in Human Spleen Tissue Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ASC antibody (2287) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human HL60 CellsLoading: 15 &956,g of lysates per lane.Antibodies: ASC 2287, (1 &956,g/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: ASC 2287, (2 &956,g/mL), ASC 39-001, (2 &956,g/mL), beta-actin (1 &956,g/mL) and GAPDH (0.02 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse (for ASC 39001) IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human THP-1 CellsLoading: 15 &956,g of lysate per lane.Antibodies: ASC 2287, (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 7 KD Validation of ASC in THP-1 Cells (Dowds et al., 2004) Immunofluorescence analysis with anti-ASC antibodies (2287) was performed for BIM in 293 cells transfected with GFP siRNA or ASC siRNA. ASC expression was disrupted after ASC siRNA knockdown.
	Figure 8 Overexpression Validation of ASC in HEK293T Cells (Dowds et al., 2004) Western blot analysis with anti-ASC antibodies (2287) was performed for ASC in HEK293T cells transfected with pcDNA3-ASC.