Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CHRFAM7A antibody (23-222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody (23-222) at dilution of 1:100 (40x lens).
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