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MDC1 Antibody, Unconjugated, Rabbit, Polyclonal

MDC1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-23-531

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Article Name:	MDC1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-23-531
Supplier Catalog Number:	23-531
Alternative Catalog Number:	PRS-23-531-100
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Conjugation:	Unconjugated
Alternative Names:	IER3, NFBD1, KIAA0170, DKFZp781A0122

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 270kDa
NCBI:	9656
UniProt:	Q14676
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (23-531) at dilution of 1:100. Blue: DAPI for nuclear staining.

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