TRPA1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-23-662
Article Name: TRPA1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-23-662
Supplier Catalog Number: 23-662
Alternative Catalog Number: PRS-23-662-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 850-950 of human TRPA1 (NP_015628.2).
Conjugation: Unconjugated
Alternative Names: TRPA1, ANKTM1, TRPA-1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 128kDa
NCBI: 8989
UniProt: O75762
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of mouse liver, using TRPA1 antibody (23-662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).