Optimal dilutions for each application to be determined by the researcher
Application Notes:
Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1)
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1)
FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1)
FFPE human pancreas tested with Histone H1 antibody (1415-1)
FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1)
FFPE human angiosarcoma tested with Histone H1 antibody (1415-1)
Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).
* VAT and and shipping costs not included. Errors and price changes excepted
