cIAP Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	cIAP Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3325
Supplier Catalog Number:	3325
Alternative Catalog Number:	PRS-3325-0.02,PRS-3325-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2. The immunogen is located within amino acids 540-590 of cIAP.
Conjugation:	Unconjugated
Alternative Names:	cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 70kDObserved: 70kD
NCBI:	329
UniProt:	Q13490
Buffer:	cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-4 µg/mL, IHC: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Human Lung LysateLoading: 15 &956,g of lysates per lane.Antibodies: cIAP 3325 (A: 1 &956,g/mL, B: 2 &956,g/mL and C: 4 &956,g/mL ), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunofluorescence Validation of cIAP in Human Lung TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
	Figure 5 Immunohistochemistry Validation of cIAP in Human Lung Tissue Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: cIAP 3325 (2 &956,g/mL), cIAP, competitor antibody, (1 &956,g/mL) and beta-actin 3779 (1.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: cIAP 3325 (2 &956,g/mL ), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Human and Rat Tissue LysatesLoading: 15 &956,g of lysates per lane.Antibodies: cIAP 3325 (1 &956,g/mL ), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunofluorescence Validation of cIAP in Human Lung TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).