ATF6 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-3683
- Images (7)
| Article Name: | ATF6 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-3683 |
| Supplier Catalog Number: | 3683 |
| Alternative Catalog Number: | PRS-3683-0.02,PRS-3683-0.1 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6. |
| Conjugation: | Unconjugated |
| Alternative Names: | ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 0.5-2 µg/mL, IHC: 5 µg/mLAntibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested. |
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Figure 2 WB Validation in Human Cell Lines Loading: 15 &956,g of human cell lysate Antibodies ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution. |
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Figure 3 WB Validation in Mouse Tissue Loading: 15 &956,g of mouse tissue lysate Antibodies: ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 5 Immunohistochemistry Validation of ATF6 in Human Testis Tissue Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 6 Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 7 Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 1 KO Validation of ATF6 Loading: 15 &956,g of human HeLa cell lysate Antibodies ATF6 3683, 0.5 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 4 WB Validation in Rat Heart Loading: 15 &956,g of rat kidney lysate Antibodies: ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. |
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