TIP47 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	TIP47 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3881
Supplier Catalog Number:	3881
Alternative Catalog Number:	PRS-3881-0.02,PRS-3881-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-TIP47 antibody (3881) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TIP47. The immunogen is located within the last 50 amino acids of TIP47.
Conjugation:	Unconjugated
Alternative Names:	TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 47kD Observed: 47kD
NCBI:	10226
UniProt:	O60664
Buffer:	TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-4 µg/mL, IHC-P: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and rat samples, Immunohistochemistry in rat samples, Immunofluorescence in rat samples. All other applications and species not yet tested.

	Figure 5 Immunofluorescence Validation of TIP47 in Rat Liver CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling TIP47 with 3881 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 4 Immunohistochemistry Validation of TIP47 in Rat Liver Tissue Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using anti-TIP47 antibody (3881) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human Daudi CellsLoading: 15 &956,g of lysates per lane.Antibodies: TIP47 3881, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 &956,g/mL , Lane2: 2 &956,g/mL
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 µg of lysates per lane.Antibodies: TIP47 3881 (4 &956,g/mL), TIP47 3883 (1 &956,g/mL), and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Rat LiverLoading: 15 &956,g of lysates per lane.Antibodies: TIP47 3881, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 &956,g/mL,Lane2: 1 &956,g/mL,Lane3: 2 &956,g/mL
	Figure 6 Induced Expression Validation of TIP47 in Atg7 Liver KO Mice (Singh et al., 2009) TIP47 expression detected by anti-TIP47 antibodies was markedly increased in the liver of Atg7 liver KO mice.
	Figure 7 Induced Expression Validation of TIP47 in Mouse Liver (Singh et al., 2009) TIP47 expression detected by anti-TIP47 antibodies was increased in the autophagic vacuoles (AVs) and lysosomes (Lys) after starvation.
	Figure 8 Induced Expression Validation of TIP47 in HT29 Cells (Qi et al., 2013) TIP47 expression detected by anti-TIP47 antibodies was increased in the colonic cells after oleic acid treatment.