Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx.17+18kDa doublet band observed in cell lysates of NIH3T3 and in Rat Adrenal Gland lysates (calculated MW of 18.8kDa according to Mouse NP_033905.3 and 18.9kDa according to Rat NP_036647.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
42-086 (3.75ug/ml) staining of paraffin embedded Human Renal Tubules. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
42-086 (3.75ug/ml) staining of paraffin embedded Human Squamous Epithelium. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. This data is from a previous batch, not on sale.
42-086 (3.75ug/ml) staining of paraffin embedded Human Adipose. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
42-086 (2ug/ml) staining of NIH3T3 (A) amd Rat Adrenal Gland (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-086 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial /cytoplasmic staining. The nuclear
42-086 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
42-086 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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