IFN-beta Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	IFN-beta Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-4243
Supplier Catalog Number:	4243
Alternative Catalog Number:	PRS-4243-0.02,PRS-4243-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-IFN-beta antibody (4243) was raised against a peptide corresponding to 17 amino acids near the center of human IFN-beta. The immunogen is located within amino acids 110-160 of IFN-beta.
Conjugation:	Unconjugated
Alternative Names:	IFN-beta Antibody: IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 22kDObserved: 22kD.
NCBI:	3456
UniProt:	P01574
Buffer:	IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 4-10 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

	Figure 6 Immunohistochemistry Validation of IFN-beta in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (4243) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Mouse A20 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: IFN-beta 4243 (Lane 1: 5 &956,g/mL and Lane 2: 10 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: IFN-beta 4243 (4 &956,g/mL), IFN-beta 64-239, (5 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Multiple Tissues and Recombinant ProteinLoading: 15 &956,g of lysates per lane.Antibodies: IFN-beta 4243 (4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Immunofluorescence Validation of IFN-beta in Human Liver CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with 4243 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 7 Immunofluorescence Validation of IFN-beta in Mouse Liver TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling IFN-beta with 4243 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 4 Immunohistochemistry Validation of IFN-beta in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (4243) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.