IL-33 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	IL-33 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-4273
Supplier Catalog Number:	4273
Alternative Catalog Number:	PRS-4273-0.02,PRS-4273-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse
Immunogen:	Anti-IL-33 antibody (4273) was raised against a 17 amino acid synthetic peptide from near the center of human IL-33. The immunogen is located within the 150-200 amino acids of IL-33.
Conjugation:	Unconjugated
Alternative Names:	IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 31kDObserved: 33kD
NCBI:	90865
UniProt:	O95760
Buffer:	IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, IF: 20 µg/mL, IHC-P: 2.5 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation of IL-33 in A20 Cells Loading: 15 &956,g of lysates per lane.Antibodies: IL-33, 4273, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 1 &956,g/mLLane B: 2 &956,g/mL
	Figure 5 Immunofluorescence Validation of IL-33 in Human Lymph NodeImmunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with 4273 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 6 Immunohistochemistry Validation of IL-33 in Human Lymph Node Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-33 antibody (4273) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Western Blot Validation of IL-33 with Human Recombinant ProteinLoading: 5ng (1) and 25ng (2) of IL-33 partial human recombinant protein.Antibodies: IL-33, 4273, 1 &956,g/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.
	Figure 3 Western Blot Validation of IL-33 in Human Lymph NodeLoading: 15. &956,g of human lymph node lysateAntibodies: IL-33, 4273, 2 &956,g/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation of IL-33 in Human LungLoading: 15. &956,g of human lung lysateAntibodies: IL-33, 4273, 2 &956,g/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 7 Induced Expression Validation of IL-33 in Human Skin Fibroblasts (Ivanov et al., 2010)Protein expression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased 1-4 h after alpha-irradiation.
	Figure 8 Regulated Expression Validation of IL-33 in Human Skin Fibroblasts (Ivanov et al., 2010)Protein expression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased after alpha-irradiation, which were inhibited by treatment of PPP.