Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.
45-219 (1ug/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-219 (2ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.This data is from a previous batch, not on sale.
45-219 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining. This data is from a previous batch, not on sale.
45-219 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
45-219 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
45-219 (5ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
45-219 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. This data is from a previous batch, not on sale.
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