This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.
Conjugation:
Unconjugated
Alternative Names:
ATP synthase subunit beta, mitochondrial, ATP5B, ATPMB, ATPSB
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5B Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using ATP5B Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of SK-BR-3 cells stained with ATP5B Antibody . AP2973c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue) .
Western blot analysis of ATP5B Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate
Western blot analysis of ATP5B using rabbit polyclonal ATP5B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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