This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
Antibody staining LAG3 in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Overlay histogram showing Jurkat cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:1000 dilution Lane 1: human ovary lysate Lane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: human liver lysates Lane 3: human ovary lysates Lysates/proteins at 20 ug per lane.
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