Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.
Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.
Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35ug/lane)
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