HMGB1 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	HMGB1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-7715
Supplier Catalog Number:	7715
Alternative Catalog Number:	PRS-7715-0.02,PRS-7715-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-HMGB1 antibody (7715) was raised against a peptide corresponding to 19 amino acids near the center of human HMGB1. The immunogen is located within 70-120 amino acids of HMGB1.
Conjugation:	Unconjugated
Alternative Names:	HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 25 kDa Observed: 25 kDa
NCBI:	3146
UniProt:	P09429
Buffer:	HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1 µg/mL, IHC: 1-2 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse, and rat samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 5 Immunofluorescence Validation of HMGB1 in HL60 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1 with 7715 at 20 &956,g /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
	Figure 6 Immunohistochemistry Validation of HMGB1 in Human Stomach Tissue Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1 antibody (7715) at 1 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7 Immunohistochemistry Validation of HMGB1 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1 antibody (7715) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 8 Immunohistochemistry Validation of HMGB1 in Rat Spleen Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1 antibody (7715) at 1 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 KO Validation in HeLa Cells Loading: 10 &956,g of HeLa WT cell lysates or HMGB1 KO cell lysates. Antibodies: HMGB1 (0.5 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 WB Validation in Human, Mouse and Rat Cell Lines Loading: 15 &956,g of lysate Antibodies: HMGB1 7715, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 WB Validation in Mouse TissuesLoading: 15 &956,g of lysate Antibodies: HMGB1 7715, 1 &956, g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 WB Validation in Rat Tissues Loading: 15 &956,g of lysate Antibodies: HMGB1 7715, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.