CD4 Antibody [9H5A8], IgG1, Unconjugated, Mouse, Monoclonal

Article Name:	CD4 Antibody [9H5A8], IgG1, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	PRS-PM-5201
Supplier Catalog Number:	PM-5201
Alternative Catalog Number:	PRS-PM-5201-0.02,PRS-PM-5201-0.1
Manufacturer:	ProSci
Host:	Mouse
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Conjugation:	Unconjugated
Alternative Names:	CD4 Antibody [9H5A8] : CD4mut

Clonality:	Monoclonal
Concentration:	1 mg/mL
Clone Designation:	[9H5A8]
Molecular Weight:	Predicted: 51kDObserved: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
Isotype:	IgG1
NCBI:	920
UniProt:	P01730
Buffer:	CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-4 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

	Figure 8 Immunohistochemistry Validation of CD4 in Human Thymus Tissue Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (PM-5201) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein.Antibodies: CD4 PM-5201 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.Observed at around 27kD.
	Figure 2 Western Blot Validation in Human Thymus Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
	Figure 3 Western Blot Validation in Human Jurkat LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
	Figure 4 Western Blot Validation in Human, Mouse and Rat Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
	Figure 5 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
	Figure 6 Western Blot Validation in Mouse Spleen Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
	Figure 7 Western Blot Validation in Rat Lung Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.