PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.
Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.
Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [4F2] (RF16031) Blue: DAPI staining
Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [4F2] (RF16031) Blue: DAPI staining
Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [4F2] (RF16031) Blue: DAPI staining
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 &956,g/mL.
Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 &956,g/mL.
Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 &956,g/ml
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