Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase.
Conjugation:
Unconjugated
Alternative Names:
ERK1,ERT2,ERK-1,PRKM3,P44ERK1
This is a p44/42 MAP Kinase(Phospho-Tyr204) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Buffer:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (RDSA62232) (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (RDSA62232).
Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (RDSA62232).
Western blot analysis of various lysates using p44/42 MAP Kinase(Phospho-Tyr204) Antibody at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution.Lysates/proteins: 25µg per lane.
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